Found insideThe main purpose of this book is to point out the interest of some important topics of tissue regeneration and the progress in this field as well as the variety of different surgical fields and operations. According to the results, the samples in the 1:2 dilution group showed relatively satisfactory cartilage regeneration in terms of homogeneity, shape maintenance, and efficiency of the regenerated cartilage. Christoph Erggelet, Congress Chair Online ahead of print. These parameters not only affect the feasibility of the future clinical translation of ECG–DBM constructs and but also play an important role in the efficiency of cartilage regeneration in vivo. Progress With Cartilage Regeneration. Debridement or Abrasion - A surgeon arthroscopically removes loose cartilage which causes bleeding at the bone surface and growth of fibrocartilage (fibrous cartilage or scar tissue ). In some cases, the fibrocartilage may not be strong enough to effectively protect the joint. A lot of information through our keynote speakers and distinguished faculty will be provided and interaction of all participants and industry is necessary to reach the goal of the meeting. BM-MSCs differentiated to chondrocytes for treatment of full-thickness cartilage defect of the knee. Reconstr. Found inside – Page 125Owing to such features, chitosan and its composites have been widely used for anti-bacterial coating, drug delivery, wound dressing, and cartilage regeneration (Mohammadzadeh Pakdel and Peighambardoust, 2018). Biochem. 63, e370–e375. In vitro regeneration of patient-specific ear-shaped cartilage and its first clinical application for auricular reconstruction. Cartilage Regeneration With Regenerative Medicine By stemxgroup | March 17, 2021 A Brief Overview of Osteoarthritis Musculoskeletal joint conditions are extremely prevalent in the US. (2010). The Opening Ceremony hosted by the ICRS Board, the ICRS awards ceremony and the ICRS Welcome cocktail will offer the best opportunities to meet with peers of the ICRS family and to join our efforts to have an outstanding meeting. Advanced technology and a design that puts healing and well-being first come together at the new Stanford Hospital — built to serve the community 100 years into the future. Here’s how it works and what an army of scientists is doing to try to end its rampage. Keywords: 3D cartilage regeneration, engineered cartilage gel, decalcified bone matrix, tissue engineering, nutrient efficiency, Citation: Ci Z, Zhang Y, Wang Y, Wu G, Hou M, Zhang P, Jia L, Bai B, Cao Y, Liu Y and Zhou G (2021) 3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix. Phys. However, the large pore size and controllable mechanical strength of DBM are precisely suitable for seeding ECG, which has a large particle size. The Young modulus of the regenerated cartilage was tested using a mechanical analyzer (Instron-5542, Instron, Canton, MA). Acta Biomater. Human cartilage sheets were prepared using a process that was similar to that used with the goat chondrocytes. The focus of this book is to create a comprehensive analysis of cartilage injury and repair strategies. Twenty chapters cover proven and emerging procedures and methodologies. Key Points Covered from the Worldwide Stem Cell Cartilage Regeneration Industry 2021 Research are: (2017). The global Cartilage Regeneration Tchnology market was million USD in 2020 and is expected to million USD by the end of 2027, growing at a CAGR of between 2021 and 2027. Standardized protocols in rehabilitation are the key for patient-related outcomes and have to be updated, especially with respect to back to sports and applied biomechanics. Found insideThis book covers the most recent developments in the field of osteochondral tissue engineering (OCTE) and covers in detail the concepts and current challenges for bone and cartilage repair and regeneration. doi: 10.1016/j.pmr.2016.06.007. New surgical techniques in various joints are technically demanding and need advice and training of how and when to use it. Gross view, HE, Safranin-O, and collagen II immunohistochemical staining of 3 d ECG–decalcified bone matrix (DBM) (A1–A8) and 15 d ECG–DBM (B1–B8) constructs. More importantly, ECG–DBM constructed from human chondrocytes also successfully regenerated mature cartilage tissue in nude mice, and 3 days ECG at a 1:2 dilution provided the optimal conditions of those tested. Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix. Zheng, R., Duan, H., Xue, J., Liu, Y., Feng, B., Zhao, S., et al. The clinical treatment of cartilage defects is a significant challenge (Gomez et al., 2020; Mardones et al., 2020; Pattappa et al., 2020), as current methods do not provide an ideal graft for repairing the cartilage defects. To prove the steel (DBM)-reinforced concrete (ECG) concept, the following scientific questions must be answered. However, we also have to look into the future and strongly move forward in the world of medical research and experimental science in cartilage repair and joint preservation. Gross (A2) and mature status (B2) images of 15 d goat cartilage sheet and gross images (C2) of 15 d ECG. Additionally, the formation method and relative parameters were investigated and optimized. Cartilage sheets were prepared as previously established (Xue et al., 2018). Biomaterials 20, 233–240. Mardones, R., Giai Via, A., Pipino, G., Jofre, C. M., Munoz, S., Narvaez, E., et al. The dependence of in vivo stable ectopic chondrogenesis by human mesenchymal stem cells on chondrogenic differentiation in vitro. doi: 10.1016/j.cbpa.2006.09.009, PubMed Abstract | CrossRef Full Text | Google Scholar, Deng, D., Liu, W., Xu, F., Yang, Y., Zhou, G., Zhang, W. J., et al. 103, 1111–1119. Welcome ICRS Virtual Convention 2021 OrthoRegenerative Therapy: From Basic Science to Clinical Application. DOI: 10.1016/j.acthis.2021.151713 Abstract Introduction: Chondroprogenitors, a promising therapeutic modality in cell-based therapy, are routinely isolated from articular cartilage … Various animal and clinical experiments have demonstrated that scaffold-free cartilage-sheet technologies can stably regenerate high-quality cartilage tissue in vivo (Li et al., 2017, 2019; He et al., 2018). J. Plast. 9:638115. doi: 10.3389/fcell.2021.638115 Biomaterials 30, 6724–6730. Reconstr. A suitable framework with controlled shape, mechanical strength, and fine pore size is required to address these problems. A simplified method for the analysis of hydroxyproline in biological tissues. Front. And as for the expression of cartilage-related genes, 15 d ECG was higher than 3 d ECG (Supplementary Figure 1). Gross view and histological examination of the cartilage regenerated by goat engineered cartilage gel (ECG) after 12 weeks in vivo in autologous goat. Histological analysis performed at 12 weeks showed that both the 3 d and 15 d ECM–DBM constructs formed mature cartilage-like tissue in the outer areas of the sample with abundant lacuna structure and homogenous cartilage ECM distribution in both nude mouse (Figures 3A2–A8,B2–B8) and autologous goat models (Figures 4A2–A8,B2–B8). At a later stage, following DBM degradation, ECG-regenerated cartilage gradually matures and finally forms intact and continuous cartilaginous tissue, which contributes to stable shape maintenance and mechanical support. Chan, PhD, and professor of surgery Michael Longaker, MD, co-senior authors of the paper. The studies involving human participants were reviewed and approved by the Weifang Medical College Ethics Committee. We look forward to welcoming all of you healthy and strong in Berlin for the 16th ICRS World Congress from April 12-15, 2022. Biomaterials 35, 152–164. Horseradish peroxidase (HRP)-conjugated anti-mouse antibody (1:200 in PBS, Santa Cruz) was then applied as a secondary antibody. Cell Dev. Schagemann, J. C., Chung, H. W., Mrosek, E. H., Stone, J. J., Fitzsimmons, J. S., O’Driscoll, S. W., et al. Mesenchymal stromal cell transplantation induces regeneration of large and full-thickness cartilage defect of the temporomandibular joint. Functional biomaterials for cartilage regeneration. 2.2.1 Global Cartilage Regeneration Market Revenue (USD Million) and Growth Rate (%) Comparison by Type (2016-2021) 2.2.2 Microfracture, Debridement, Abrasion Arthroplasty, Autologous Chondrocyte Implantation, Osteochondral Autograft Transplantation, Osteochondral Allograft Transplantation, Cell Based Cartilage Resurfacing, Others Surg. Primer sequences of related genes. These findings suggested that appropriate ECG dilution is vital for satisfactory cartilage regeneration using ECG–DBM constructs. This study established a new concept for cartilage regeneration—analogous to steel-reinforced concrete—based on ECG and DBM and demonstrated its feasibility. Online or onsite, Lucerne, Switzerland ON Foundation info@on-foundation.org true MM/DD/YYYY. Total glycosaminoglycan (GAG) (D) and total collagen (E) in different groups after 12 weeks in vivo implantation (in vivo). Although cartilage-sheet technology is a promising strategy for cartilage regeneration, an uncontrolled shape and poor mechanical strength greatly hinder further clinical application (Li et al., 2017, 2019). 15:455. Both 3 d and 15 d ECGs had fine ECG adhesion efficiency (Figures 2D,E). Pattappa, G., Krueckel, J., Schewior, R., Franke, D., Mench, A., Koch, M., et al. Found insideThis volume outlines the current status in the field of biomimetic medical materials and illustrates research into their applications in tissue engineering. Found insideIn addition, various aspects related to the testing and characterisation of fibres and polymers are reviewed. This book is an invaluable resource to students, lecturers, industrial technologists and researchers in this subject area. … Meng, Q., Man, Z., Dai, L., Huang, H., Zhang, X., Hu, X., et al. Stem Cells Int. 2021 May 17;S0168-3659(21)00231-5. doi: 10.1016/j.jconrel.2021.05.015. Therefore, the current study investigated a novel mode of 3D cartilage regeneration based on the design concept of steel (DBM)-reinforced concrete (ECG). “ Cartilage Regeneration Market ” Report 2021 covers strong research on global industry size, share and growth which enables the customer to look at … The global regenerative medicine for cartilage market is expected to grow from $4.47 billion in 2020 to $4.83 billion in 2021 at a compound annual growth rate (CAGR) of 8.10%. “One idea is to follow a ‘Jiffy Lube’ model of cartilage replenishment,” Longaker said. J. Tissue Eng. 24, 147–150. Biol. The results indicated that ECG cultured in vitro for 3 days (3 d) and 15 days (15 d) showed good biocompatibility with DBM, and the ECG–DBM constructs successfully regenerated viable 3D cartilage with typical mature cartilage features in both nude mice and autologous goats. Auricular cartilage was obtained from autologous goat and cut into 1.0 mm pieces, washed using phosphate-buffered solution (PBS), and digested using 0.15% collagenase (Worthington Biochemical Corp., NJ, United States) for isolating chondrocytes, as previously reported (Rodriguez et al., 1999). Quantitative analysis of the wet weight (A), volume (B), and volume change rate (C) in different groups after 3-day culture in vitro (in vitro) and 12 weeks in vivo (in vivo). Schinhan, M., Gruber, M., Dorotka, R., Pilz, M., Stelzeneder, D., Chiari, C., et al. In this study, cartilage sheet was prepared into engineered cartilage gel (ECG) and combined with DBM to explore the feasibility of regenerating 3D cartilage with controlled shape and mechanical strength. (2018). Three 6-month-old goats (Shanghai Jiagan Biological Technology Co., Shanghai, China) were used in this study. The patients/participants provided their written informed consent to participate in this study. Reconstr. The chondrocytes were then cultured in chondrogenic medium comprising DMEM, 10 ng/ml of TGF-β1 (R&D Systems Inc. Minneapolis, MN, United States), 40 ng/ml of dexamethasone (Sigma-Aldrich, St. Louis, MO, United States), 100 ng/ml of IGF-I (R&D Systems Inc. Minneapolis, MN, United States), 1% insulin-transferrin-selenium-linoleic acid (ITS, ScienCell, CA, United States), and 1% antibiotic-antimycotic (Gibco BRL) for 3 and 15 days. (2018). The current study provides a new cartilage regeneration mode and predicts its potential in future clinical translation. Intercontinental Berlin Berlin, Germany. decisivemarketsinsights August 11, 2021. 23 ECG at this dilution ratio had a relatively low viscosity, which led to ECG loss in the outer areas of the construct because of the low viscosity and low adhesion efficiency after seeding, which resulted in inferior cartilage regeneration in the outer areas. Mater. SAN DIEGO, June 30, 2021 (GLOBE NEWSWIRE) -- Histogen Inc. (NASDAQ: HSTO), today announced the initiation of its Phase 1/2 clinical trial of HST 003 to evaluate the safety and efficacy of human extracellular matrix (hECM:HST 003) implanted within microfracture interstices and the cartilage defect in the knee to regenerate hyaline cartilage in combination with a microfracture … Surg. Acta Biomater. Autologous cartilage transplantation is currently the most effective method for treating cartilage defects; however, there is limited source tissue, and the potential exists for irreversible damage to the donor area (Schinhan et al., 2013; Ruta et al., 2016). (3) Does the ECG need to be diluted, and if so, what proportion is optimal? 2022-04-12. However, some important issues, including how to precisely control the shape and realize homogeneity of the whole regenerated cartilage, still need to be investigated. Stable subcutaneous cartilage regeneration of bone marrow stromal cells directed by chondrocyte sheet. *P < 0.05. However, because of its large pore size and the difficulty in controlling its uniformity, DBM readily loses cells after seeding and cannot be directly used for cartilage regeneration (Man et al., 2016; Xu B. et al., 2017). Would you like to send us a note or receive a print edition? We look forward to welcoming all of you healthy and strong in Berlin for the 16th ICRS World Congress from April 12-15, 2022. Reviewing exhaustively the current state of the art of tissue engineering strategies for regenerating bones and joints through the use of biomaterials, growth factors and stem cells, along with an investigation of the interactions between ... Found inside – Page iiiThis book employs a wealth of high-quality illustrations to provide the reader with a detailed understanding of the anatomy and the histology of the cartilage, the etiology and the classification of the cartilage lesions, and the numerous ... doi: 10.1016/s0142-9612(98)00165-3. Engineering cartilage substitute with a specific size and shape using porous high-density polyethylene (HDPE) as internal support. 2021-10-02. On a mission to survive, the coronavirus leaves behind a trail of destruction. Figure 2. Res. The 3 d ECG was prepared from 3 days cartilage sheets that were collected directly using a 5 ml syringe and mixed well with the 1.5-fold chondrogenic medium. The global Cartilage Repair/Regeneration market size is projected to reach USD 820 million by 2027, from USD 486.5 million in 2020, at a CAGR of 7.7% during 2021-2027. This volume covers all aspects of embryonic stem cell differentiation, including mouse embryonic stem cells, mouse embryonic germ cells, monkey and human embryonic stem cells, and gene discovery. * Early commitment steps and generation of ... (2010). GW, MH, PZ, LJ, and BB: relative data analyses. September 7, 2021 Complete study of the global Cartilage Regeneration Tchnology market is carried out by the analysts in this report, taking into consideration key factors like drivers, challenges, recent trends, opportunities, advancements, and competitive landscape. DBM is an ideal natural tissue regeneration scaffold with biocompatibility and immunogenicity, controllable shape, and mechanical strength that can be controlled by the degree of decalcification (Zheng et al., 2004; Liese et al., 2013; Meng et al., 2015; Xie et al., 2017). Matrix-associated autologous chondrocyte transplantation in a compartmentalized early stage of osteoarthritis. *P < 0.05. Copyright © 2021 Ci, Zhang, Wang, Wu, Hou, Zhang, Jia, Bai, Cao, Liu and Zhou. Ge, Z., Li, C., Heng, B. C., Cao, G., and Yang, Z. This work is the result of a partnership that began in 2011, when I received for the first time the invitation to be the scientific editor of a book on bone grafting, by the still little publisher known as InTech. doi: 10.1016/j.actbio.2019.03.003, Li, D., Zhu, L., Liu, Y., Yin, Z., Liu, Y., Liu, F., et al. Aug 17 2020 Register data and long term outcomes can set the stage for the evaluation of existing technologies. The optimal ECG dilution for cartilage regeneration efficiency in future clinical applications was also evaluated. “You don’t wait for damage to accumulate — you go in periodically and use this technique to boost your articular cartilage before you have a problem.”. Briefly, all samples (n = 5 per group) were weighed using an electronic balance. (2015). Found insideRegenerative medicine – stem cell and gene-based therapy – offers a new approach for restoring function of damaged organs and tissues. This is the first book to cover the major new aspects and field of regenerative medicine. Loss of this slippery and shock-absorbing tissue layer, called articular cartilage, causes joint pain and arthritis, which afflicts more than 55 million Americans. RT-qPCR was performed according to the manufacturer’s protocol (Thermo Fisher Scientific, Waltham, MA, United States). After many years of increasing our knowledge and insights into cartilage repair and joint preservation, we have to go back on one hand to critically analyse and learn from our successes and failures in regeneration and repair and to rethink what happened. Isolated chondrocytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM) medium (Gibco BRL, Grand Island, NY, United States) containing 10% fetal bovine serum (Gibco BRL) and 1% antibiotic-antimycotic (Gibco BRL). The animal study was reviewed and approved by the Weifang Medical College Ethics Committee. Repair of articular cartilage defects with acellular cartilage sheets in a swine model. Found insideThis book focuses on cartilage defects and new mesenchymal stem cell-based treatments for their repair and regeneration. Early chapters provide a review of current etiological findings and repair methods of cartilage defects. Tissue-engineered trachea regeneration using decellularized trachea matrix treated with laser micropore technique. The chondrocyte sheets cultured for 3 days were only preliminarily formed and were relatively immature. Scaffold-free cartilage-sheet technology can stably regenerate high-quality cartilage tissue in vivo. TUNEL staining confirmed that a large number of apoptotic cells were detected in the inner areas but not in the outer areas (Figures 6C1–C3,F1–F3), which implies that the immature cartilage/fibrous-like tissue in the inner areas probably derived from implanted ECG, whose cells were dying because of nutrient deficiency caused by the continuous cartilage-like tissue on the periphery of the implant blocking nutrient transport. Data Bridge Market Research August 18, 2021. It goes on to discuss the impact of these developments on the market’s overall economic growth. Regeneration of trachea graft with cartilage support, vascularization, and epithelization. Found insideThis book covers our current understanding of the role of mesenchymal stem cells (MSCs) and other mesenchymal progenitors in skeletal regeneration, encompassing bone, cartilage and whole joint regeneration. The total RNA was extracted from 3 d and 15 d ECGs (n = 3 per group), and cDNA was obtained by reverse transcription (RT) according to a previously described method (Jiang et al., 2010). The DNA content of the ECG–DBM framework at different times (E). A 93, 454–463. International Cartilage Regeneration & Joint Preservation Society ( ICRS ) Virtual Convention 2021 was conducted to outline key strategies frequently used in research and clinical application, including blood -derivatives, stem c ells, biomechanical concepts and engineering strategies, as The Cartilage Regeneration Market report comprises reviews about key players within the market, major collaborations, merger and acquisitions. Found insideIn Mercies in Disguise, acclaimed New York Times science reporter and bestselling author Gina Kolata tells the story of the Baxleys, an almost archetypal family in a small town in South Carolina. Found inside – Page 76The thought that intra- articular injections of stem cells can help improve/restart chondrogenesis, and regeneration of damaged cartilage is actively being researched. However, a recent systematic review of the literature shows only a ... Found inside – Page iiiThis book serves as an anatomic atlas of the nerves that innervate the joints of the human body in a format that also provides technical insight into pathways that both interventional pain management and surgical subspecialists can use to ... Additionally, because of its rapid degradation (ten Koppel et al., 1998; van Osch et al., 1999), DBM cannot be used alone as a tissue regeneration scaffold, which greatly limits its application in the field of cartilage regeneration. Med. 13:025016. doi: 10.1088/1748-605x/aa99a4. As previously described (Liu et al., 2008), samples from different groups (n = 6 per group) were cut into 4 mm-diameter cylinders. Research of joint omeostasis and ageing will bring new aspects of treatment modalities in degenerative joint diseases. This issue of Stanford Medicine magazine examines racial inequity and inequality in medicine and explores initiatives to close the gaps in care. 12, 1123–1132. 4 modules on 2 afternoons (Wednesdays), 90 min duration per module. (2012). Additionally, some immature cartilage-like tissue (15 d) and fibrous-like tissue (3 d) were observed in the inner areas of all the samples (likely because of nutrient deficiency). Coculture of allogenic DBM and BMSCs in the knee joint cavity of rabbits for cartilage tissue engineering. Cell Dev. ECG at this dilution ratio had a high viscosity, which led to local accumulation on the surface of the construct liquidity that caused excessive proliferation and deformation on the surface. N-3-12 = the cartilage regenerated by 3 d ECG–decalcified bone matrix (DBM) constructs after 12 weeks in vivo in nude mice; N-15-12 = the cartilage regenerated by 15 d ECG–DBM constructs after 12 weeks in vivo in nude mice; G-3-12 = the cartilage regenerated by 3 d ECG–DBM constructs after 12 weeks in vivo in autologous goats; G-15-12 = the cartilage regenerated by 15 d ECG–DBM constructs after 12 weeks in vivo in autologous goats. Sci. N. Am. Extracellular vesicle-functionalized decalcified bone matrix scaffolds with enhanced pro-angiogenic and pro-bone regeneration activities. Physioxia expanded bone marrow derived mesenchymal stem cells have improved cartilage repair in an early osteoarthritic focal defect model. After confirming the suitable mature status of ECG, the optimal ratio of ECG dilution was the next key parameter. Decalcified bone matrix (DBM) has high porosity, a suitable pore structure, and good biocompatibility, as well as controlled shape and mechanical strength. 2021 Feb 12;2021:8882505. doi: 10.1155/2021/8882505. Experimental study on allogenic decalcified bone matrix as carrier for bone tissue engineering. Poly-epsilon-caprolactone/gel hybrid scaffolds for cartilage tissue engineering. The stress–strain curves were obtained from the first 40%. Stem Cell Cartilage Regeneration Market Size (sales, revenue) forecast by regions and countries from 2021 to 2026 of Stem Cell Cartilage Regeneration … Obstacles for clinical use of those successful technologies have to be discussed among clinical researchers, scientists, and industry to develop strategic measurements for the implementation of these treatments for the best of our patients. The 15 d ECG was prepared from 15 days cartilage sheets that were minced into pieces (Figure 1C2). Chem. While some of these are being used today, researchers continue to look into new ways to regrow cartilage in an attempt to give people relief from the pain of osteoarthritis. Quantitative evaluations of human engineered cartilage gel–decalcified bone matrix (ECG–DBM) constructs. Outside the box thinking about injury recovery, mental and physical fitness. The feasibility of cartilage regeneration using ECG–DBM constructs was the first key point in the current study. Found insidePrimarily focused on the cardiovascular applications where there have been the greatest advancements toward the clinic, this is the first compendium for clinical and biomedical researchers who are interested in integrating MSC-derived ... The role of trabecular demineralized bone in combination with perichondrium in the generation of cartilage grafts. (2010). Now Available On-Demand (if you have pre-registered and paid your fee, you can login with your ICRS ID (to be found on your receipt) and your registered Last Name.All sessions are available for on-demand view until end of September 2021 (2008). Additionally, both 3 d and 15 d ECGs combined with DBM regenerated favorable cartilage tissue in vivo. Magali Cucchiarini & Stefan Nehrer, Scientifc Programme Chairs, +41 (0)44 503 73 70 Gomez, M., Wittig, O., Diaz-Solano, D., and Cardier, J. E. (2020). To isolate the chondrocytes, the cartilage pieces were washed by PBS and digested by 0.15% collagenase (Worthington Biochemical Corp., Freehold, NJ, United States) as previously described (Deng et al., 2009). Abandoned cartilage tissue was donated by patients. The pandemic of musculoskeletal diseases – like osteoarthritis – and the burden of pain and immobility will still challenge our daily work in research and clinics. As shown in Figure 6, both the outer cartilage-like tissue and inner immature cartilage/fibrous tissue showed negative CD3 (Figures 6A1–A3,D1–D3) and CD68 expression (Figures 6B1–B3,E1–E3), which indicates that no host inflammatory cells participated in the cartilage regeneration.

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