We also provide details for testing the activity in mammalian cells of custom TALE-TFs and TALENs using quantitative reverse-transcription PCR and Surveyor nuclease, respectively. View details for PubMedCentralID PMC3684555. Join the Wu Tsai Neurosciences Institute Mailing List, Diversity, Inclusion, Belonging, Equity and Justice. Their combined citations are counted only for the first article. It could be engineered to function in eukaryotic cells as a fast, low-cost, efficient, and scalable tool for manipulating genomic sequences. We further identify Gata-3, a zinc-finger transcription factor in the dysfunctional module, as a regulator of dysfunction, and we use CRISPR-Cas9 genome editing to show that it drives a dysfunctional phenotype in CD8(+) TILs. The model was experimentally validated for sgRNA-mediated mutation rate and protein knockout efficiency. ", Biology (School of Humanities and Sciences) (Phd Program). Found inside – Page 14720 , 50 ; FSS - 9 , v.c. , Le Havre , Feb. ... 12 , 1914 ; Stanford U. , A.B. 1937 ; Central U. of Venezuela , Caracas , 1939–40 ; sec . to Am . minister to El Salvador 1934–35 ; dep . collector , Bu . of Internal Revenue , 1937-39 ... Transcription activator-like effectors (TALEs) are a class of naturally occurring DNA-binding proteins found in the plant pathogen Xanthomonas sp. Le Cong 丛乐 Stanford University, Stanford School of Medicine Verified email at stanford.edu. Found inside – Page 10631972 ; Procureur auprès de la cour mun . de Montréal 1973–76 ; Substitut du Procureur - Gén . pour le Min . de la ... Cong . de la Société de criminologie du Qué .; Charge de cours ( tribunal école ) à la fac . de droit , Univ . de ... Bio-X Affiliated Faculty. Le Cong. of Chemical Engineering, focuses on the synthesis of functional organic and polymer materials, organic electronic device design and fabrication, and applications for organic electronics. We validated our REDIT approach using different formats and lengths of knock-in templates. Dr. Lei Stanley Qi is Assistant Professor in the Department of Bioengineering, Department of Chemical and Systems Biology, and a faculty fellow in Stanford ChEM-H. Check Stanford University – Find it@Stanford in the search results, then click Save. RNA profiles are an informative phenotype of cellular and tissue states but can be costly to generate at massive scale. The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes and enables direct testing of the causal roles of genetic and epigenetic regulation in normal biological processes and disease states. For exceptions to this generalization see Haas, Ernst, Human Rights and International Action, (Stanford: Stanford University Press, 1970)Google Scholar, and Emerson, Rupert, “ The Fate of Human Rights in the Third World,” World Politics, Vol. These tumors are highly hypoxic but poorly vascularized, suggestive of deficient angiogenesis signaling. 27, No. Luan, B., Xu, G., Feng, M., Cong, L., Zhou, R. IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development. View details for Web of Science ID 000352454600031, View details for PubMedCentralID PMC4393360. "United States Patent 8,932,814 CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes. Found inside – Page 323Awards : Fel , Huntington Hartford , 64 & Guggenheim , 67 & 72 ; Res Scholar , Rockefeller Found , Bellagio , Italy , 73 ; two Anton ... Study : Harvard Univ , BA , 65 ; Univ Calif , Berkeley , MA , 67 , PhD , 69 ; Stanford Univ , 68. Aditya Kunjapur ... Clinical Assistant Professor at Stanford University. San Francisco Bay Area. Found inside – Page 102Le. Himmelheber , Joseph Bernard . — b . Annapolis , Md . , Dec. 16 , 1902 ; Loyola High Sch . ( Baltimore ) grad . ; U. of Md . ... B.A. 1937 ; Columbia , M.A. 1938 , grad . residence scholar 1937-39 , Butler scholar 1938– 39 ; app . "United States Patent 8,906,616 Engineering of systems, methods and optimized guide compositions for sequence manipulation", Le Cong, Feng Zhang. 1, Atlanta, March 2011, pp. View details for DOI 10.1093/nar/gkaa1264. PRIME: A novel processing-in-memory architecture for neural network computation in ReRAM-based main memory. Davies, Mill wrote: I submit, that he who saves another from drowning in order to kill him by torture afterwards, does not differ only in motive from him who does the same thing from duty or benevolence; the act itself is different. To gain a deeper molecular understanding of the dysfunctional T cell state, we analyzed population and single-cell RNA profiles of CD8(+) tumor-infiltrating lymphocytes (TILs) and used genetic perturbations to identify a distinct gene module for T cell dysfunction that can be uncoupled from T cell activation. These data suggest that microRNAs promote tumor responses to hypoxia and angiogenesis by repressing FIH1. Our experiments inhibiting repair enzyme activities suggested that REDIT has the potential to overcome limitations of endogenous DNA repair steps.Finally, our REDIT method is applicable across cell types including human stem cells,and is generalizable to different Cas9 enzymes. Interleukin-17 (IL-17)-producing helper T cells (Th17 cells) play an important role in autoimmune diseases. We systematically assessed the DNA sequence features that contribute to single guide RNA (sgRNA) efficiency in CRISPR-based screens. Le Cong 丛乐 Stanford University, Stanford School of Medicine Verified email at stanford.edu. The dynamic nature of gene expression enables cellular programming, homeostasis and environmental adaptation in living systems. ", Feng Zhang, LeCong, Randall Platt, Neville Sanjana, Fei Ann Ran. Google Scholar; Ping Chi, Shuangchen Li, Cong Xu, Tao Zhang, Jishen Zhao, Yongpan Liu, Yu Wang, and Yuan Xie. Advanced Techniques in Molecular Neuroscience, View details for DOI 10.1089/crispr.2021.29124.lec. The Zhenan Bao Research Group at Stanford University, Dept. RBPJ binds and trans-activates the Il23r promoter and induces IL-23R expression and represses anti-inflammatory IL-10 production in Th17 cells. Found inside – Page 132( Also , at New Brunswick , in Child , Francis James , scholar , b . in Boston . bier is produced by the Manuel Garcia June , Jacob Broadhead , president , and ... editor , b . in Md . Beecher , Thomas K. , Cong . cl . , born in Conn . Tested on independent data sets, the model achieved significant results in both positive and negative selection conditions and outperformed existing models. Removing one or more exons from the mutated transcript can produce an in-frame mRNA and a truncated, but still functional, protein. Nidetz, N. F., McGee, M. C., Tse, L. V., Li, C., Cong, L., Li, Y., Huang, W. Take Risks and Constantly Challenge the Status Quo, Combined Computational-Experimental Approach to Explore the Molecular Mechanism of SaCas9 with a Broadened DNA Targeting Range. These studies often utilize fluorescent protein tags to facilitate live cell imaging. Xu, H. n., Xiao, T. n., Chen, C. H., Li, W. n., Meyer, C. A., Wu, Q. n., Wu, D. n., Cong, L. n., Zhang, F. n., Liu, J. S., Brown, M. n., Liu, X. S. Global microRNA depletion suppresses tumor angiogenesis. A structural comparison of SaCas9 with SpCas9 highlighted both structural conservation and divergence, explaining their distinct PAM specificities and orthologous sgRNA recognition. We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications. We thus find that Notch signaling influences the development of pathogenic and non-pathogenic Th17 cells by reciprocally regulating IL-23R and IL-10 expression. Found inside – Page 793Leland Stanford Estes Second book of tales . Field , E. $ 1.25 . ... Le Queux , W : $ 1 . ... .Cong . Pub Shinn , G : Wolfe . Friendly talks about marriage . 97 Schineinitz , G. E. de . Diseases of the eye . 2d rev . [ '96 . ) ... Google Scholar See Mkhitarian, Suren A., Rabochii Klass i Natsional' no-Osvoboditel' noe Dvizhenie vo Vietname (The Working Class and the National-Liberation Movement in Vietnam) (Moscow, 1967), p. 153. Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more than 1 kilobase shorter. View details for PubMedCentralID PMC4509999. The TALE toolbox described here will enable a broad range of biological applications. View details for PubMedCentralID PMC5726792. Found inside – Page 252Author : Le Theatre du People a Paris , 1885-1914 , 1949. Ph.D. Dissertation : ( See Author ) . Travel : France ; Germany ; Austria ; Mex .; England . Gen. Int .: Dean's Scholar , Columbia Univ . , 1931-32 . Neville Sanjana Core Faculty, New York Genome Center. Dr. Cong is a recipient of the NIH/NHGRI Genomic Innovator Award, a Baxter Foundation Faculty Scholar, and has been selected by Clarivate Web of Science as a Highly Cited Researcher. Sanjana, N. E., Cong, L. n., Zhou, Y. n., Cunniff, M. M., Feng, G. n., Zhang, F. n. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. In this chapter, detailed protocols are described for harnessing the CRISPR-Cas9 system from Streptococcus pyogenes to enable RNA-guided genome engineering applications in mammalian cells. On Chiang Kai-shek's view, see Lin, H.-T. (2009). 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 1989 1988 1987 1986 1985-1978 . 8 Grossman, Herschel I., “Kleptocracy and Revolution,” Oxford Economic Papers 51, no. Hoang-Quynh Le (Lê Hoàng Quỳnh) Lecture at VNU University of Engineering and Technology Verified email at vnu.edu.vn Zhengxuan Wu Stanford University Verified email at stanford.edu Mai-Vu Tran (Trần Mai Vũ) Phòng thí nghiệm Công nghệ tri thức, Đại học Công nghệ, Đại … Dissection of causal gene functions in cellular and organismal processes therefore necessitates approaches that enable spatially and temporally precise modulation of gene expression. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. Found inside – Page 562U.S.A , 54-57 , 1st Lt. AHA ( Beveridge Award Comt , 73-75 ) ; Conf . ... Tex , 64 ; La lucha por la consolidacion de la nacionalidad Argentina , 1852-1862 , Hachette , Buenos Aires , 64 ; Representantes diplomaticos britanicos y estado ... Journal of Materials Chemistry A 1 (15), 4754-4762. , 2013. Journal of Materials Chemistry A 4 (19), 7107-7134. , 2016. His group has a focus on using these tools to study immunological and infectious diseases. Adil Akkouch, PhD. View details for DOI 10.1126/science.aad5177, View details for Web of Science ID 000368440500046, View details for PubMedCentralID PMC4924477. 2017 Dr. Cong's Lab Homepage. Assistant Professor of Pathology (Pathology Research) and of Genetics, PhD, Harvard University, Harvard Medical School., Biological and Biomedical Sciences (2014), LHB, Harvard Medical School., Certificate in Leder Human Biology and Translational Medicine, B.S., Tsinghua University, Biological Sciences, Electronic Engineering (2009). Le Cong Assistant Professor at Stanford University School of Medicine San Francisco Bay Area. Dark Paradise tells the story not only from the standpoint of legal and medical sources, but also from the perspective of addicts themselves."--BOOK JACKET. Customized TALEs can be used for a wide variety of genome engineering applications, including transcriptional modulation and genome editing. Dr. Cong's research group at the Department of Pathology and Department of Genetics is developing technology for genome editing and single-cell genomics, using computational approaches inspired by data science. These functional in vivo analyses revealed an unexpectedly complex landscape of tumor suppression that has implications for understanding cancer evolution, interpreting clinical cancer genome sequencing data, and directing approaches to limit tumor initiation and progression. Journal of Management Studies, 45 (8), 1401–1423. Merged citations. Le Cong Assistant Professor at Stanford University School of Medicine San Francisco Bay Area. Found inside – Page 323Awards : Fel , Huntington Hartford , 64 & Guggenheim , 67 & 72 ; Res Scholar , Rockefeller Found , Bellagio , Italy , 73 ; two Anton ... Study : Harvard Univ , BA , 65 ; Univ Calif , Berkeley , MA , 67 , PhD , 69 ; Stanford Univ , 68. In vivo genome editing using Staphylococcus aureus Cas9. Crossref, Google Scholar; Marx, K and F Engels [1902] Wage-labor and Capital. Bio. Nature Biotechnology 2011). Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana. These methods are excellent for studies of monomeric proteins, but for multimeric proteins, they are unable to rule out artifacts from native protein subunits already present in the cells. Here we use a multiplexed mouse model of oncogenic KRAS-driven lung cancer to quantify the impact of forty-eight known and putative tumor suppressor genes on diverse aspects of carcinogenesis at an unprecedented scale and resolution. Transcription activator-like effectors are sequence-specific DNA-binding proteins that harbour modular, repetitive DNA-binding domains. Compositions and methods of treating head and neck cancer. B.S., Tsinghua University, Biological Sciences, Electronic Engineering (2009) View Stanford profile. LITEs do not require additional exogenous chemical cofactors, are easily customized to target many endogenous genomic loci, and can be activated within minutes with reversibility. We further assess the genome-wide targeting specificity of SaCas9 and SpCas9 using BLESS, and demonstrate that SaCas9-mediated in vivo genome editing has the potential to be efficient and specific. View details for Web of Science ID 000287023000022, View details for PubMedCentralID PMC3084533, Genomic Innovator Award, National Institute of Health (NIH), National Human Genome Research Institute (NHGRI), Donald and Delia Baxter Foundation Faculty Scholar, Baxter Foundation, CRI Irvington Fellow, Cancer Research Institute, HHMI International Fellow, Howard Hughes Medical Institute, Genome Editing and New Investigator Committee Member, American Society of Gene & Cell Therapy (ASGCT) (2019 - Present), PhD, Harvard University, Harvard Medical School., Biological and Biomedical Sciences (2014), LHB, Harvard Medical School., Certificate in Leder Human Biology and Translational Medicine, B.S., Tsinghua University, Biological Sciences, Electronic Engineering (2009), Feng Zhang, Le Cong, Patrick Hsu, Fei Ann Ran.

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